Experiences, such as environmental enrichment (EE), that allow for exploration often lead to brain changes and alter novelty-seeking behaviors, and adolescence is a developmental period in which these behaviors increase. In this study, the effects of EE during adolescence on preference for familiar objects that have been rearranged using an object-in-place (OiP) task and on neural activation in the hippocampus, which is implicated in the detection of novel spatial relationships, was investigated. Adolescent Long-Evans rats (n=16) were exposed to EE between postnatal days (PND) 25 and 48.