The problem of resistance to β-lactam antibiotics has become widespread. When the antibiotics were first developed, most strains of bacteria were susceptible, but, due to over- prescription, many strains today have developed resistance. The aim of this project is to create sensing systems to monitor the hydrolysis of β-lactam antibiotics. These systems will utilize β-lactamase, a gene responsible for antibiotic resistance, and enhanced green fluorescent protein (EGFP), a pH sensitive fluorescent protein.